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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP1034a
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WB, IHC, E
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H
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RB1852
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_072046, Q9Y6K1
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human Dnmt3a.
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Other Names:
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DNA (cytosine-5)-methyltransferase 3A; Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA
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Application Data:
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Calculated MW:
101558 Da
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Western blot analysis of anti-Dnmt3a Pab (Cat. #AP1034a) in T47-D cell lysate. Dnmt3a (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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- Koturbash, Igor, et al. Carcinogenesis 2007 Aug 01;28(8):1831-1838. (Applications: WB)
- Tryndyak, Volodymyr P., et al. Carcinogenesis 2006 Aug 01;27(8):1713-1720. (Applications: WB)
- I Koturbash, et al. Oncogene.2006 Jul 20;25(31):4267-75. (Applications: WB)
- Pogribny, Igor, et al. Mol. Cancer Res. 2005 Oct 01;3(10):553-561. (Applications: WB)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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